理研セミナー

高度好熱菌 丸ごと一匹 プロジェクト
第9回 連携研究会

2010年8月20日(金) ~ 8月22日(日)



はじめに


1~10.Progress in the whole-cell project of an extreme thermophile,Thermus thermophilus HB8

Synchrotron-Radiation System Biology Research Group
(RIKEN SPring-8 Center)


11.Publication of experimental data in crystallographic analysis of bacterial proteins by integrated database project

Naoki Kunishima, Akina Ozawa, Michihiro Sugahara, Akeo Shinkai, Seiki Kuramitsu,Yukuhiko Asada
(RIKEN Harima SPring-8 Center)


12.Integrated database resources in Kyoto Encyclopedia of Genes and Genomes (KEGG) and Database Center for Life Science (DBCLS)

Toshiaki Katayama
(Human Genome Center, Institute of Medical Science, University of Tokyo)


13.Biological resources of thermophiles Thermus thermophilus, Aeropyrum pernix and Sulfolobus tokodaii in the Gene Engineering Division, RIKEN BioResource Center

Takehide Murata, Masato Okubo, Shotaro Kishikawa, Yukari Kujime, Chitose Kurihara,Koji Nakade, Megumi Hirose, Satoko Masuzaki, Yuichi Obata
(Gene Engineering Division, RIKEN BioResource Center)


14.Identification of small non-coding RNAs in Thermus thermophilus

Gota Kawai1,2, Nobuharu Ito1, Akito Shimada1,Seiki Kuramitsu2,3, Gen-ichi Sampei2,4, Yoshitaka Bessho2
1Chiba Inst. Tech., 2RIKEN Harima Inst, 3Osaka Univ., 4Univ. of Electro-Commun.)


15.Possible missing genes of Thermus thermophilus HB8 genome

Shin-ichi Yokobori1, Tairo Oshima2, Yoshitaka Bessho3
1Dept. Mol. Biol., Sch. Life Sci., Tokyo Univ. Pharm. Life Sci., 2Kyowa Kako, 3RIKEN SPring-8)


16.Mobile genetic elements of Thermus thermophilus: a functional insertion sequence and novel phages

Masatada Tamakoshi1,2, Momoko Okuda1, Motoki Sugisawa1, Akihiko Yamagishi1
1Dept. Mol. Biol., Tokyo Univ. Pharm. Life Sci., 2RIKEN SPring-8 Center, Harima Institute)


17.Strategies for Expression of Foreign Genes in Thermus thermophilus : The Case Study of the Deinococcal Tryptophan Synthase Gene

Yoshinori Koyama
(AIST, BPRI)


18.Building a Database for Deinococcus and Thermus Comparative Genomic Analyses

Kei Yura
(Center Info. Biol., Ochanomizu Univ)


19.Proteogenomics of bacteria: from genome annotation to physiology

Jean Armengaud
(CEA, DSV, iBEB, Lab Biochim System Perturb, France)


20.New era for network biology of Escherichia coli

Hirotada Mori1,2
1Graduate School of Biological Sciences, Nara Institute of Science and Technology, 2Advanced Institute of Biosciences, Keio University)


21.Nucleic Acids (DNA and RNA) are not boring long polymers composed of only four types of nucleotides: evolutionary implications

Henri GROSJEAN
(University of Paris‐South and CNRS UMR 8621, Institute of Genetics and Microbiology, France)


22.New micro-beam beamline at SPring-8, targeting at protein micro-crystallography

Masaki Yamamoto1, Kunio Hirata1, Go Ueno1, Yoshiaki Kawano1, Takaaki Hikima1, Atsushi Nisawa1, Hironori Murakami1, Nobutaka Shimizu1,2, Takashi Kumasaka1,2
1RIKEN SPring-8 Center,.2SPring-8/JASRI)


23.Development of Coherent Imaging Methods Using Synchrotron Radiation and FEL

Y. Nishino1, Y. Tanaka2, Y. Takahashi3, K. Maeshima4, M. Guizar-Sicairos5, Y. Bessho2, T. Oroguchi6, Y. Joti7, M. Okada8, M. Okaya9, K. Nozaki10, R. Tsutsumi3, M. Yabashi7, M. Nagasono7, S. Matsui8, J. Fienup5, K. Yamauchi3, E. Matsubara9, and T. Ishikawa2,7
1 Hokkaido Univ., 2SPring-8/RIKEN, 3Osaka Univ., 4NIG, 5Univ. of Rochester, 6Yokohama City Univ., 7XFEL/RIKEN, 8Univ. of Hyogo, 9Kyoto Univ., 10Kwansei Gakuin Univ.)


24.Recent activity of neutron diffractometers for biological crystallography at JAEA

Taro Tamada, Kazuo Kurihara, Takashi Ohhara, Ryota Kuroki
(Quantum Beam Science Directorate, Japan Atomic Energy Agency (JAEA))


25.Integration of Imagings Using Electrons and Photons

Kuniaki Nagayama
(Okazaki Inst. for Integrative Bioscience & National Inst. for Physiol. Sciences)


26.The X-Ray Free Electron Laser in Japan

Tetsuya Ishikawa
(RIKEN Harima Institute)


27.Challenges regarding the 3D imaging of biological macromolecules in the gas phase - Preparing for x-ray free electron laser experiments

J. Günter Grossmann1, Takaaki Hikima2, James A. Austin1, Andrew Entwistle3, Masaki Yamamoto2, Richard W. Strange1, Naoto Yagi2, Ikuo Konishi3, Tetsuya Ishikawa2, S. Samar Hasnain1
1Molecular Biophysics Group, Faculty of Health and Life Sciences, University of Liverpool; 2RIKEN SPring-8 Center; 3Shimadzu Research Laboratory, Manchester)


28.The System-Biological Project of a Model Organism, Thermus thermophilus HB8: Frontier Light for the Central Dogma of Life

Yoshitaka Bessho1, Akeo Shinkai1, Seiki Kuramitsu1,2
11RIKEN SPring-8 Center. 2Osaka University)


29.Study of transcriptional regulatory system of T. thermophilus HB8: Regulatory mechanism of CRISPR/cas system

Akeo Shinkai
(RIKEN SPring-8 Center, Harima Inst.)


30.Structural and functional analysis of the global transcriptional regulator SdrP from Thermus thermophilus HB8

Yoshihiro Agari1,2, Seiki Kuramitsu1,2,3, Akeo Shinkai1
1RIKEN Spring-8 Center, Harima Inst., 2Grad. Sch. of Fronteir Biosci., Osaka Univ., 3Grad. Sch. of Sci., Osaka Univ.)


31.Structure and function of the transcriptional repressor CsoR from Thermus thermophilus HB8

Keiko Sakamoto1, Yoshihiro Agari1, Kazuko Agari1, Seiki Kuramitsu1,2, Akeo Shinkai1
1RIKEN SPring-8 Center, Harima Inst. 2Grad. Sch. of Sci., Osaka Univ. )


32.“Intracellular thermosensor” function of a cold shock protein from Thermus thermophilus HB8

Ryosuke Mega1, Akeo Shinkai2, Noriko Nakagawa1,2, Ryoji Masui1,2, and Seiki Kuramitsu1,2
1Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2RIKEN SPring-8 Center)


33.Global analysis of RNases from Thermus thermophilus HB8

Hiromasa Ohyama1, Noriko Nakagawa1,2, Seiki Kuramitsu1,2, and Ryoji Masui1,2
1 Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2 RIKEN SPring-8 Center, Harima Inst.)


34.Structural comparison of β-CASP nucleases: RNase J and CPSF groups

Hirohito Ishikawa1, Hiromasa Ohyama1, Yuya Nishida2, Noriko Nakagawa1,3, Seiki Kuramitsu1,2,3, Ryoji Masui1,3
1 Grad. Sch. Sci., Osaka Univ., 2Grad. Sch. Frontier Biosci., Osaka Univ., 3RIKEN SPring-8 Center, Harima Inst.)


35.Network of modified nucleosides and modification enzymes in Thermus thermophilus tRNA

Chie Tomikawa1, Kazuo Ishida1, Takashi Kunibayashi1, Anna Ochi1, Tamotsu Kanai2, Takashi Yokogawa3, Akira Hirata1, Chikako Iwashita1, Hiroyuki Hori1,4,5
1Grad. Sch. of Eng. and Sci., Ehime Univ., 2Grad. Sch. of Eng., Kyoto Univ., 3Fac. of Eng., Gifu Univ., 4Venture Business Lab., Ehime Univ., 5 RIKEN Harima Inst., SPring-8)


36.Establishment of in vitro assay system for folate-dependent tRNA U54 methyltransferase TrmFO

Ryota Yamagami1, Koki Yamashita1, Hiroshi Nishimasu2, Chikako Iwashita1,Akira Hirata1,Osamu Nureki2, Hiroyuki Hori1,3
1Dept. of Materials Sci. and Biotechnol., Grad. Sch. Of Sci. and Eng., Ehime Univ.,2Dept. of Basic Biophysic.and.Biochem.,Grad.Sch.of.Sci., Univ. of Tokyo,3VBL, Ehime Univ)


37.Functional analysis of dihydrouridine synthetase from Thermus thremophilus

Takeshi Yoshida1, Eri Iwasaki1,Takako Awai1,Chie Tomikawa1, Akira Hirata1,2, Hiroyuki Hori1,2
1Dept. of Materials Sci. and Biotechnol., Grad. Sch. of Sci. and Eng., Ehime Univ.,2VBL, Ehime Univ)


38.The importance of a modified nucleoside m7G46 in 3D-core of tRNA in Thermus thermophilus

Chie Tomikawa1, Tamotsu Kanai2, Takashi Yokogawa3, Hiroyuki Hori1,4,5
1Grad. Sch. of Sci. and Eng., Ehime Univ., 2Grad. Sch. of Eng., Kyoto Univ. 3Fac. of Eng., Gifu Univ., 4Venture Business Lab., Ehime Univ., 5RIKEN Harima, SPring-8)


39.Biosynthesis of tRNA thiouridine in Thermus thermophilus and its evolutionary relationship with eukaryotic ubiquitin system

Naoki Shigi1, Yuriko Sakaguchi2, Shin-ichi Asai3, Tsutomu Suzuki2, Kimitsuna Watanabe4
1 BIRC, AIST, 2 Grad. Sch. Eng., Univ. of Tokyo, 3JBIC, 4Tokyo Univ. of Pharm. and Life Sci.)


40.Whole-cell proteome analysis of T. thermophilus HB8 by using nano-LC and Q-TOF

Kwang KIM1, Yoshio TAKAHATA2, Ryoji MASUI1,3, and Seiki KURAMITSU1,2,3
1Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2Grad. Sch. Frontier Biosciences, Osaka Univ., 3RIKEN SPring-8 Center, Harima Institute)


41.Analysis of protein phosphorylation in prokaryotes –Identification of phosphorylated protein by mass spectrometry–

Yoshio Takahata1, Kwang Kim2, Ryoji Masui2,3, Seiki Kuramitsu1,2,3
1Grad. Sch. Frontier Biosci., Osaka Univ., 2Grad. Sch. Sci., Osaka Univ., 3RIKEN SPring-8 Center, Harima Inst.)


42.Genetic analysis of the gcp gene, an unknown-essential gene common to living organisms

Kazumasa Kitahara1, Takefumi Onodera2, Takayuki Hoshino1, Issay Narumi2, Akira Nakamura1
1Grad. School of Life Environ. Sci, Univ. Tsukuba, 2 Quantum Beam Science Directorate, JAEA)


43.Structural and functional analysis of a highly conserved hypothetical protein, TTHA1606

Fumiaki Tomoike1, Taisuke Wakamatsu1, Noriko Nakagawa2,3, Ryoji Masui2,3, and Seiki Kuramitsu1,2,3
1Grad. Sch. Frontier Biosci., Osaka Univ., 2Grad. Sch. Sci., Osaka Univ., 3RIKEN SPring-8 Center, Harima Inst.)


44.Structural and functional analysis of a putative novel nucleotide-metabolizing enzyme, TTHA1091

Akiko Tsunetou1, Fumiaki Tomoike2, Noriko Nakagawa1,3, Ryoji Masui1,3, Seiki Kuramitsu1,2,3
1Grad. Sch. Sci., Osaka Univ., 2Grad. Sch. Frontier Biosci., Osaka Univ., 3RIKEN SPring-8 Center, Harima Inst)


45.Thermus thermophilus HB8 periplasmic proteins TthA0610, TthA1422 and their redoxmediated disulfide bond formation

Takashi Tamura, Takamasa Yoshida, Asako Kiyoto, Akio Ebihara, Seiki Kuramitsu, and Kenji Inagaki
(Dept of Bioscience, Grad. Sch. Nat. Sci. & Tech., Okayama Univ.)


46.The thermodynamic effect of a designed disulphide bond on the molecular surface of cold shock protein from Thermus thermophilus HB8

Shn-ichi Kidokoro, Aoi Hasegawa, Naoto Hirose,Takashi Miyamoto,Rena Sato
(Nagaoka University of Tech.)


47.Crystal structure of a novel goose-type lysozyme with chitinase activity from Ralstonia sp. A-471

Takao Arimori1, Nobuo Okazaki1, Mitsuhiro Ueda2, Masami Nakazawa2, Kazutaka Miyatake2, Ryota Kuroki1, Taro Tamada1
1JAEA, 2Osaka prefecture Univ.)


48.Photoresponse of Thermus thermophilus HB27

Hideaki Takano1, Ryuta Yamazaki1, Wataru Hakamada1, Akira Nakamura2, Takayuki Hoshino2, Teruhiko Beppu1, Kenji Ueda1
1Nihon Univ., 2Univ. of Tsukuba)


49.Responses of Cell to Stress –Analysis of Heat Shock Protein from System Biology-

Nakayama Hiroko1, Hosono Kiyoshi1,Isozaki Kenji1, Akio Ito2,Akira Yanou3,Meisou To3,Yusuke Murase4,Kazuhiko Yamamoto1,2
1Kinki Univ. Grad., 2Kinki Univ. Engin., 3Okayama Univ., 4Shudo Univ.)


50.Structural Studies on General Stress Response Mechanism in Bacillus subtilis

Len Ito, Masatomo Makino, Takeshi Hoshino, Nobutaka Shimizu, Takashi Kumasaka
(JASRI/SPring-8)


51.Formation of semiconductor nano-particles by bacteria isolated from natural oil-producing environments

Toshifumi Sakaguchi, Ryosuke Shimizu
(Prefectural Univ. of Hiroshima)


52.Enigmas of Polyamine Metabolisms of Thermus thermophilus HB8: Is sym-Homospermidine Synthesized by a Unique Enzyme?

Tairo Oshima
(Institute of Environmental Microbiology, Kyowa-kako)


53.Lysine and arginine biosynthesis in Sulfolobus

Takuya Ouchi1, Takeo Tomita1, Akira Horie1, Tomohisa Kuzuyama1, Makoto Nishiyama1,2
1Biotechnology Research Center, The University of Tokyo, 2RIKEN SPring-8)


54.Molecular mechanism for allosteric regulation of glutamate dehydrogenase from Thermus thermophilus

Takeo Tomita1, Makoto Nishiyama1,2
1 Biotechnology Research Center, The University of Tokyo, 2RIKEN SPring-8)


55.Mechanism of Feedback Inhibition of Homocitrate Synthase from Thermus thermophilus

Takuya Okada1, Takeo Tomita1, Tomohisa Kuzuyama1, Makoto Nishiyama1,2
1Biotechnology Research Center, The University of Tokyo, 2RIKEN SPring-8)


56.The crystal structures of amino acid kinase LysZ and carrier protein LysW involved in lysine biosynthesis of Thermus thermophilus

Ayako Yoshida, Takeo Tomita, Tomohisa Kuzuyama, Makoto Nishiyama
(Biotechnology Research Center, The University of Tokyo)


57.Multilateral analysis of purine and pyrimidine nucleotides biosynthetic pathway

Gen-ichi Sampei1,2, Ryota Miyazawa1,Hideya Yamamoto1, Sakiko Suzuki3,Seiki Baba2,4, Noriko Nakagawa2,5, Akio Ebihara2, and Gota Kawai2,3
1University of Electro-Communications, 2RIKEN Harima Institute, 3 Chiba Institute of Technology, 4Japan Synchrotron Radiation Research Institute, 5Osaka University)


58.Crystal structure of PurK from Thermotoga maritima

Ryota Miyazawa1, Mayumi Kanagawa2, Seiki Baba2,3, Noriko Nakagawa2,4, Akio Ebihara2, Gota Kawai2,5, Gen-ichi Sampei1,2
1 University of Electro-Communications, 2RIKEN Harima Institute, 3Japan Synchrotron Radiation Research Institute, 4Osaka University, 5Chiba Institute of Technology)


59.An unique metabolism of pyrimidine nucleosides in Thermus thermophilus HB8

Fumiaki Tomoike1, Noriko Nakagawa2,3, Ryoji Masui2,3, and Seiki Kuramitsu1,2,3
1 Grad. Sch. Frontier Biosci., Osaka Univ., 2Grad. Sch. Sci., Osaka Univ., 3RIKEN SPring-8 Center)


60.Neutron Diffraction Study of ADP-ribose pyrophosphatase from Thermus thermophilus HB8

Motoyasu Adachi1, Takao Arimori1, Taro Tamada1, Kazuo Kurihara1, Takushi Ooga2, Seiki Kuramitsu2, Nobuo Kamiya3,Ryota Kuroki1
1QuBS JAEA, 2Osaka Univ., 3Osaka City Univ.)


61.DNA repair subsystems of Thermus thermophilus HB8

Kenji Fukui1, Ryoji Masui1,2, Noriko Nakagawa1,2, Taisuke Wakamatsu3, Hitoshi Iino1, Rihito Morita2, Masao Inoue2, Atsuhiro Shimada2, Shuhei Nakane2, Kyoko Majima2, Yuya Nishida2, Ryuichi Asano2, Seiki Kuramitsu1,2,3
1 RIKEN・Harima Inst., 2Grad. Sch. Sci. Osaka Univ., 3Grad. Sch. Frontier Biosci.)


62.A general mechanism for SSB displacement from ssDNA upon SSB–protein interaction

Jin Inoue1,2, Takayuki Nagae3, Masaki Mishima3, Yutaka Ito3, Takehiko Shibata1,2,Tsutomu Mikawa1,2,4
1 RIKEN Adv. Sci. Inst., 2Grad. School of Nanobioscience, Yokohama City Univ., 3Dept. Of Chem., Tokyo Metropolitan Univ., 4RIKEN SPring-8 center)


63.Direct interaction of RecO with RecA is involved in regulation of RecA function

Takeshi Shinohara1, Jin Inoue1,2, Takehiko Shibata1,2, Tsutomu Mikawa2,3
1Graduate School of Nanobioscience Department of Supramolecular Biology, Yokohama City University, 2RIKEN Advanced Science Institute, 3RIKEN Spring-8 Center)


64.Analysis of the inhibition mechanism of extension of RecA filament by RecX

Saya Okui1,2, Jin Inoue1,2, Kunitake Iju1, Kaori Habu3, Yutaka Ito3, Takehiko Shibata1,2,Tsutomu Mikawa1,2,4
1Grad. School of Nanobioscience, Yokohama City Univ., 2RIKEN Adv. Sci. Inst., 3Dept. Of Chem., Tokyo Metropolitan Univ., 4RIKEN SPring-8 center)


65.Cellular and molecular functional analysis of a bacterial Rad52_Rad22 family protein from Thermus thermophilus HB8

Tatsuhiko Inoue1, Atsuhiro Shimada2, Noriko Nakagawa2,3, Ryoji Masui2,3, Seiki Kuramitsu1,2,3
1Grad. Sch. Frontier Biosci., Osaka Univ., 2Grad. Sch. Sci., Osaka Univ., 3RIKEN SPring-8 Center, Harima Inst.)


66.Inactivation of DNA repair genes leads to activation of vitamin B1 biosynthesis in Thermus thermophilus HB8

Kenji Fukui1, Taisuke Wakamatsu1,2, Yoshihiro Agari1,2, Ryoji Masui1,3, Seiki Kuramitsu1,2,3
1RIKEN SPring-8 Center, 2Grad. Sch. Sci. Osaka Univ., 3Grad. Sch. Frontier Biosci.)


67.Structural and functional analyses of ATP-dependent regulation of MutL

Hitoshi Iino1,2, Kwang Kim2, Tatsuya Yamamoto3, Atsuhiro Shimada2, Ryoji Masui1,2, Seiki Kuramitsu1,2, Kenji Fukui1
1RIKEN SPring-8 Center, 2Grad. Sch. of Sci. Osaka Univ., 3Keio Univ. Sch. of Med.)


68.Structure of RecJ exonuclease definces its specificity for single-stranded DNA

Taisuke Wakamatsu1, Yutaro Kotera2, Noriko Nakagawa2,3, Seiki Kuramitsu1,2,3, Ryoji Masui2,3
1Grad. Sch. Frontier Biosci., Osaka Univ., 2Grad. Sch. Sci., Osaka Univ., 3RIKEN SPring-8 Center, Harima Inst.)


69.Role of RecJ-like 5'-3' exonuclease in oligo(deoxy)nucleotide degradation

Taisuke Wakamatsu1, Kwang Kim2, Noriko Nakagawa2,3, Seiki Kuramitsu1,2,3, Ryoji Masui2,3
1Grad. Sch. Frontier Biosci., Osaka Univ., 2Grad. Sch. Sci., Osaka Univ., 3RIKEN SPring-8 Center, Harima Inst.)


70.The partial reconstitution of DNA mismatch repair in Thermus thermophilus HB8

Atsuhiro Shimada1, Kenji Fukui2, Noriko Nakagawa1,2, Ryoji Masui1,2, Seiki Kuramitsu1,2
1Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2RIKEN SPring-8 Center, Harima Inst.)


71.Molecular functional analysis of RadA/Sms: A RecA-like protein

Masao Inoue1, Kenji Fukui2, Noriko Nakagawa1,2, Ryoji Masui1,2, and Seiki Kuramitsu1,2
1Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2RIKEN SPring-8 Center, Harima Inst.)


72.Functional analysis of an alkyltransferase-like protein by DNA microarray

Hisahiro Hishinuma1, Rihito Morita1, Noriko Nakagawa1,2, Ryoji Masui1,2, Seiki Kuramitsu1,2
1Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2RIKEN SPring-8 Center)


73.Functional analysis of an alkyltransferase-like protein that involved in a novel nucleotide excision repair system

Rihito Morita1, Nakagawa Noriko1,2, Seiki Kuramitsu1,2, Ryoji Masui1,2
1Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2RIKEN SPring-8 Center, Harima Inst.)


74.DNA-Protein Binding Studies: Investigating an ATL Protein from T.thermophilus HB8

Oliver Wilkinson1, David M. Williams1, Ryoji Masui2, Seiki Kuramitsu2
1Centre for Chemical Biology, University of Sheffield, UK, 2Graduate School of Science, Osaka University)


75.Molecular functional analysis of DNA polymerase I in base excision repair

Kyoko Majima1, Shuhei Nakane1, Noriko Nakagawa1,2, Ryoji Masui1,2, Seiki Kuramitsu1,2
1Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2RIKEN SPring-8 Center, Harima Inst.)


76.Structural and functional analysis of bacterial DNA polymerase X involved in DNA repair processes

Shuhei Nakane1, Hirohito Ishikawa1, Noriko Nakagawa1,2, Ryoji Masui1,2, Seiki Kuramitsu1,2
1Dept. Biol., Grad. Sch. Sci., Osaka Univ., 2RIKEN SPring-8 Center, Harima Inst.)


77.Functional analysis of nucleoid associated protein HU from Thermus thermophilus HB8

Yuya Nishida1, Noriko Nakagawa2,3, Ryoji Masui2,3, Seiki Kuramitsu1,2,3
1Grad. Sch. Frontier Biosci., Osaka Univ., 2RIKEN SPring-8 Center, Harima Inst., 3Grad. Sch. Sci., Osaka Univ.)


78.An additional C-terminal loop in endonuclease IV, an apurinic/apyrimidinic endonuclease, controls binding affinity to DNA

Ryuichi Asano1, Hirohito Ishikawa1, Shuhei Nakane1, Noriko Nakagawa1,2, Seiki Kuramitsu1,2, Ryoji Masui1,2
1Dept. Biol. Sci., Grad. Sch. Sci., Osaka Univ., 2RIKEN SPring-8 Center, Harima Inst. )